The Mallotus species (Euphorbiaceae) are used in traditional medicine for different ailments ranging from minor infections such as gastrointestinal disorders to dysentery, hepatic diseases, cutaneous diseases, fever and malaria, and a series of other indications. M. paniculatus is distributed in southeastern Asia and China. Previous investigations on the constituents of M. paniculatus have isolated several compounds such as cardenolides, triterpenoids, steroids, flavonoids, and unsaturated fatty acids, and the plant extract was found to possess antioxidant and antiradical activities. To find further active principles from M. paniculatus, we investigated the plant. M. paniculatus was collected from Xishuangbanna of Yunnan, China in October 2009. The air-dried stems were extracted with 95% EtOH, and the resulting solution was partitioned with CHCl3, EtOAc, and n-BuOH successively. The EtOAc extract was separated by silica gel column chromatography and Sephadex LH-20 column chromatography. Eight steroidal compounds (1-8) were isolated and identified. Compounds 1, 2, 3, and 5 were isolated from the Mallotus genus for the first time, whereas compounds 4 and 8 were originally isolated from M. paniculatus.