A glycosidic fraction was extracted from fresh cherry leaves and treated with commercial β-glucosidase and the acetone powder from cherry leaves. After enzymatic hydrolysis, the formation of moderately antifungal benzaldehyde, benzyl alcohol, 2-phenylethanol and coumarin was confirmed by GLC and GC-MS. A survey of the constituents in the glycosidic fraction revealed the presence of benzyl β-D-glucoside, 2-phenylethyl β-D-glucoside, and mandelonitrile β-D-glucosides (sambunigrin and prunasin). It was confirmed that these glucosides are possible precursors of antifungal substances in cherry leaves, which would be hydrolyzed by endogenous hydrolytic enzymes to release antifungal substances when the leaves are damaged. Although the precursor of coumarin has not yet been detected, these glucosides are stored in intact plant tissues and hydrolyzed by endogenous enzymes to antifungal substances when exposed to pathogens, classifying them as "post-inhibitins" that contribute to the defense role in plants.