Two novel aminooligosaccharides, butytatins M03 and M13 were isolated and purified from the culture filtrate of Streptomyces luteogriseus. Analysis by liquid chromatography coupled to electrospray ionization mass spectrometry indicated their resemblance to isovalertatin, with a four-carbon acyl group. Their structures were established by NMR as aminooligosaccharide derivatives possessing a butylated side chain.