The plant Sophora alopecuroides L. (family Legumineosae), which is rich in alkaloids, has been widely studied [1-7]. From 4 kg of the air-dry plant (collected in the village of Toktagul, Kirghiz SSR, in August, 1976) by extraction with methanol we have isolated 60 g of total bases (yield 1.5%). From a benzene extract of the combined material (22 g), by chromatography on a column (alumina, activity grade II, 50:1, eluents chloroform and chloroform-methanol (100:1, 100:5, 10:1, 2:1, and 1:1)) we have isolated sophoridine (0.51%), dehydrosophoridine (0.0075%), matrine (0.12%), sophocarpine (0.15%), neosophoramine (0.06%), sophoramine (0.08170%), aloperine (0.045%), cytisine (0.0075%), and methylcytisine (0.005%). From a chloroform extract (6 g) by column chromatography (cellulose (1:1) with butan-1-ol-acetic acid-water (100:15:27) (system I) as eluent) we have isolated sophoridine N-oxide (0.23%), matrine N-oxide (0.09%), and sophocarpine N-oxide (0.16%). The mother liquor after the extraction of the combined alkaloids with benzene and chloroform was evaporated to dryness in a rotary evaporator. The dry residue was treated with methanol. When the methanolic fraction of the mother liquor was separated on a column of cellulose (system I), new bases were isolated: (8) with mp 59-60°C, [α]D²⁰ --18.9° (c 0.98; ethanol), M⁺ 338, and (9) with M⁺ 296. The mass spectrum of base (8) had the peak of the molecular ion M⁺ 338 and ions characteristic for lupinine alkaloids, with the maximum intensity ion at m/e 138 indicating a transquinolizidine system (confirmed by a Bohlmann band in the IR spectrum). The IR spectrum also contained strong absorption bands at 1150 and 1170 cm⁻¹ corresponding to a β fragment, at 1524 cm⁻¹ corresponding to free NH bonds, and at 1605 and 1683 cm⁻¹ corresponding to the stretching vibrations of a secondary amide group (--NH-CO-). In the 3080 and 3300 cm⁻¹ regions there are bands characteristic for the stretching vibrations of an associated NH bond of a secondary amide group. In the PMR spectrum of base (8) there is a broadened one-proton triplet at 6.08 ppm. A two-proton triplet at 3.95 ppm has the splitting constant J = 6 Hz, indicating a --OCH₂--CH₂-- fragment. Another two two-proton multiplets are located at 2.9-3.4 and 3.62 ppm (the latter characteristic for H₂e and H₃e protons of lupinine derivatives). A three-proton triplet at 0.89 ppm shows a --CH₂CH₂CH₃ chain. These spectral characteristics suggest a lupinine skeleton for base (8) (partial structure I). Base (9) also has a lupinine skeleton and differs from base (8) only by the substituent R = C₅H₉O₂. Thus, new alkaloids having a lupinine skeleton have been isolated from S. alopecuroides L. Anabasine hydrochloride has been recommended by the Pharmacological Committee of the Ministry of Public Health of the USSR for manufacture and wide medicinal use. Its starting material is N-nitrosoanabasine, isolated from technical anabasine sulfate by nitrosation [1]. The presence of N-nitrosoanabasine in the pharmacopoeial preparation must be avoided. Chromatographic analysis of N-nitrosoanabasine in anabasine hydrochloride using a nonfixed alumina layer was described in a provisional pharmacopoeial paper [2], but it is inconvenient (free-flowing alumina) and has low sensitivity (500 μg for N-nitrosoanabasine). No publications exist on thin-layer chromatography of anabasine hydrochloride using a fixed silica gel layer. Thus, we optimized the solvent system and deposited substance amount. Using KSK silica gel (2 g) with gypsum (0.1 g) and distilled water (8 ml) on 50×200 mm glass plates (activated 24 h at room temperature), 125 μg of methanol solution was spotted. Ascending chromatography was performed in an 80×200 mm cylindrical chamber, visualized with Dragendorff reagent. Tested solvent systems: 1) chloroform-methanol (2:1), 2) chloroform-methanol-acetone (25:7:3), 3) ether-methanol (50:1), 4) benzene-methanol-acetone (10:5:2). System 2 gave the best separation: anabasine hydrochloride (Rf 0.35) and N-nitrosoanabasine (Rf 0.92). Minimum detectable amounts: 1 μg for anabasine hydrochloride and 1.2 μg for N-nitrosoanabasine. Our developed thin-layer chromatographic method is recommended for inclusion in the draft Pharmacopoeia paper and Provisional Pharmacopoeial paper as a purity test for anabasine hydrochloride.