Phytochemical studies on the ethanolic extract of the roots of Buxus sempervirens L. of Turkish origin have resulted in the isolation of four new steroidal alkaloids, (+)-16R-hydroxy-Nabenzoylbuxadine [(20S)-16R-hydroxy-3â-(benzoylamino)-20-(dimethylamino)-9,10-seco-buxa-9(11),1(10)-diene] (1), (+)-semperviraminone [3â-(dimethylamino)-20-oxo-9,10-seco-buxa-9(11),1(10),16(17)-triene] (2), (+)-Na-demethylsemperviraminone [3â-(methylamino)-20-oxo-9,10-secobuxa-9(11),1(10),16(17)-triene] (3), and (+)-buxaminol-C [(20S)-16R-hydroxy-3â-(methylamino)-20-(dimethylamino)-9,10-seco-buxa-9(11),10(19)-diene] (4). Four known steroidal alkamines, (-)-(E)-cyclobuxaphylamine [(20E)-16-oxo-3â-(methylamino)-9â,10â-cyclobuxa-7(8),17(20)-diene] (5), (-)-(Z)-cyclobuxaphylamine [(20Z)-16-oxo-3â-(methylamino)-9â,10â-cyclobuxa-7(8),17(20) diene] (6), (+)-cyclomicrobuxeine [3â-(dimethylamino)-20-oxo-9â,10â-cyclobuxa-4(30),16(17) diene] (7), and (+)-papilamine [(20S)-3â,20-bis(methylamino)-9,10-seco-buxa-9(11),10(19)-diene] (8), were also isolated for the first time from this species. Their structures were elucidated on the basis of extensive spectroscopic studies.