During previous studies, fuzanins A–D, new carbamate- and pyridine-containing compounds, were isolated from Kitasatospora sp. IFM10917. Herein, further investigation of the culture of this strain led to the isolation of five new carbamate-containing metabolites, fuzanins E–I (1–5), from its extract. The compounds were purified via ethyl acetate partitioning, silica gel column chromatography, Sephadex LH-20 column chromatography, and reversed-phase HPLC on ODS. Their structures, including stereochemical configurations, were elucidated using spectroscopic techniques (high-resolution fast atom bombardment mass spectroscopy, infrared spectroscopy, 1H/13C nuclear magnetic resonance, 1H-1H correlation spectroscopy, heteronuclear multiple bond correlation, nuclear Overhauser effect) and the modified Mosher's method. Bioactivity assays revealed that fuzanins E–H (1–4) did not inhibit Wnt signal transcription activity at 100 μM in a luciferase reporter gene assay, and fuzanins E–I (1–5) showed no antimicrobial activity against Bacillus subtilis at 100 μg per disc.