Introduction: Recently there has been a shift in universal trend from synthetic to herbal medicine, which we can say 'Return to Nature'. Cassia fistula or golden shower plant has plenty of medicinal values. This paper represents the isolation of two new chemical entities present in the leaves of Cassia fistula and their medicinal aspects. Methods: Leaves of Cassia fistula were used as plant material. Extraction was done with methanol followed by isolation procedure using HPTLC. Confirmation of compounds was done using UV, FTIR, NMR and MS spectroscopy. In vitro antibacterial screening was carried out by disc diffusion method. Results: Air-dried powdered leaves of Cassia fistula were extracted using methanol. From this extract, two compounds were isolated in their pure form using column chromatography and HPTLC. Identification of these two isolated compounds was confirmed by physicochemical data, spectral interpretation and elemental analysis. Conclusions: This is the first report of the isolation of the new compounds from Cassia fistula leaves extracted in methanol extract. These compounds are seen to exhibit moderate antimicrobial property.