A new 13,14-seco-16,24-cyclosteroid, physalin L, has been isolated, along with known compounds, from Physalis minima. The known compounds were physalin B, epoxyphysalin B and physalin D. The structure elucidation of physalin L showed it to be 6β-ethoxy-5α,13β,20β,22-tetrahydroxy-1,4,5-dioxo-16,24-cyclo-13,14-seco-ergost-2-ene 18,24-dioic acid, 18-20,27-22 dilactone.