Four new cyclic depsipeptides have been isolated from the entomopathogenic fungus Metarhizium anisopliae. The chemical structures of destruxins A3 (4) and F (5) and desmethyldestruxins A (6) and C were determined based on nmr and mass spectral data. Destruxin A3 bears an N-methylalanine moiety in place of the usual proline amino acid, while destruxin F contains a new 2,4-dihydroxypentanoic acid subunit. Spectral data for destruxin E diol [SI, previously reported as a metabolic product of destruxin E, are also presented.