A new C19-diterpenoid alkaloid, 1-epi-16β-hydroxycardiopetaline, together with 19 known diterpenoid alkaloids, was isolated from the roots of Aconitum vilmorinianum Kom. The structure of the new alkaloid was established on the basis of spectral data (HR-MS, X-ray crystallographic analysis, 1D- and 2D-NMR).