Streptomyces pluracidomyceticus PA-41746 was found to produce additional β-lactam components besides the previously reported pluracidomycins (PLM) A, B and C. Fermentation at 38°C resulted in an increase of the minor components with a decrease of PLM C, while the yields of PLM A and PLM B remained almost unchanged. The isolation and structure elucidation of these minor pluracidomycins were reported. New components PLM A₂, C₂, C₃, and D were isolated from the fermentation filtrate via phase-transfer extraction, ion-exchange chromatography on QAE-Sephadex, gel filtration, and column chromatography (MCI GEL CHP20P, HPLC). PLM A₂, identified as the first microbial metabolite bearing a sulfinic acid group (confirmed by ¹H/¹³C NMR, mass spectrometry (SIMS) showing molecular formula C₉H₁₀O₇NS₂Na₂, and oxidation to PLM A₁), differs from PLM A₁ only in the C-3 substituent. PLM C₂ is presumed to be a sulfated olivanic acid with a sulfinyl group at C-3 based on UV and ¹H NMR data. PLM C₃ was deduced as the sulfoxide of MM 17880 via spectral comparisons. PLM D, a desulfated PLM A₁ analog, shows good stability at neutral pH. These minor pluracidomycins also exhibited broad antibacterial and β-lactamase inhibitory activities.