A Japanese Loganiaceae plant, Gardneria multiflora, has been found to be a rich source of indolic alkaloids, 1) and the structure of the main base, gardneramine was fully elucidated by us. 2,3) This paper deals with the isolation and structure elucidation of four new alkaloids of this plant. The structure of cooccurring novel dimeric indole alkaloid, gardmultine, is to be published elsewhere. 5) The crude base obtained by methanol extraction of this plant was roughly fractionated by Al203 column chromatography. As examination of a gardneramine fraction by TLC revealed coexistence of three minor bases, they were isolated by the use of preparative TLC (Silicagel GF254 (Merck), acetone-CHCl3 4:5 as a developer). The fastest moving base (II) was crystallised from acetone to give colorless prisms, C22H24O5N2·1/2 H2O, mp.159-160°, [α]D -248° (MeOH). Its spectral data and chemical tests showed this base is a gardneramine analogue with a methylene dioxyl and a methoxyl group on its aromatic ring instead of the three methoxyl groups of I, named gardfloramine. The second base (III), C22H26O4N2, mp160-161.5°, [α]D -147°, was deduced to be 18 desmethoxy gardneramine (III) with E configuration for the Δ19 double bond. The third base (IV) was obtained in only a minor amount as an amorphous powder, proved to be 18-desmethoxy gardfloramine (IV). A few years ago we reported the isolation of a new alkaloid with a tentative name of alkaloid F from the same plant. This base (V), now named chitosenine after the Japanese name of this plant "Chitose-kazura", has been elucidated to possess a structure with molecular formula C22H28O6N2, a 7-spiro oxindole basic skeleton with three methoxyl groups at 9,10 and 12 positions, an α glycol system at C(16) and C(17). Its gross skeleton and absolute configuration was finally confirmed by the conversion to an iminoether (IX) whose UV, IR, NMR and CD spectra were superimposable to that of gardneramine (I) whose absolute configuration is known.