<jats:title>Significance</jats:title> <jats:p>Cyclodextrins are cyclic oligosaccharides used in the pharmaceutical industry to improve drug delivery. Here, we show that cyclodextrins improve the antifungal activity of the anabaenolysins A and B lipopeptides and that are both produced by the same cyanobacteria. This study identifies the putative biosynthetic gene cluster involved in the synthesis of these unique cyanobacterial lipopeptide anabaenolysins.