The phytochemical analysis of a butanolic fraction of Acmella ciliata Kunth. afforded a total of 15 hydrophilic compounds. Seven flavonol 3-O-glycosides were isolated, including two new natural products, quercetin-3-O-(3-O-acetyl-β-D-glucuronopyranoside) and quercetin-3-O-(2-O-acetyl-α-L-rhamnopyranosyl-(1 → 6)-β-D-glucopyranoside). Furthermore, a variety of caffeoylquinic acid derivatives could be identified using ESI‑MS and NMR-spectroscopy.