Antitumor antibiotics rhodilunancins A and B were isolated from a culture of Streptomyces violaceus var. lunanensis var. n. No. 1289 by acidic acetone extraction of mycelia and purified by silica gel chromatography. Rhodilunancin A is identical to cosmomycin A. Rhodilunancin B is identical to cosmomycin B (= rhodomycin Y). Both antibiotics exhibited activities against Gram-positive bacteria and inhibition on DNA synthesis of P388 leukemia cell in vitro.