In our search for natural antitumor compounds, we have extracted the previously uninvestigated Allamanah scbottii Pohl (Apocynaceae). The genus Allamanah is known to produce bioactive (antitumor, algicidal, and antimicrobial) iridoids (1-3) and in a recent report has been shown to contain coumarins (4). Interest in the bioactive iridoids has led to papers outlining synthetic methodology (5,6) and their distribution in the Apocynaceae (7,B) as well as the Clionidae (the boring sponges) (9). We report here the isolation from this plant of seven previously known compounds. Brine shrimp (BS) lethality was used as a monitor in the bioactivity-directed fractionation (10). The active components, in order of elution on Si gel, are: isoplumericin, plumericin, scoparone, allamandin, scopoletin, pinoresinol, and allamcin. The brine shrimp assay is a simple indicator of cytotoxicity and expedites the isolation of a wide range of bioactive compounds (in this case iridoids, coumarins, and a lignan). This assay correlates well with 9KB (human nasopharyngeal carcinoma) results. Antitumor activity was detected by the potato disc assay, which measures the inhibition of crown gall tumors on potato discs inoculated with Agrobacterium tumefaims (11). This assay shows excellent agreement with 3PS (P-388 murine leukemia) results. The brine shrimp and potato disc assays are suggested as convenient supplements to the traditional 9KB and 3PS antitumor assays (12), sparing the need for higher animals or their serum (Table 1).