Time-Resolved Transcriptomics and Constraint-Based Modeling Identify System-Level Metabolic Features and Overexpression Targets to Increase Spiramycin Production in Streptomyces ambofaciens

Time-Resolved Transcriptomics and Constraint-Based Modeling Identify System-Level Metabolic Features and Overexpression Targets to Increase Spiramycin Production in Streptomyces ambofaciens Effects of glucose limitation on biomass and spiramycin production by Streptomyces ambofaciens Resistance to spiramycin in Streptomyces ambofaciens, the producer organism, involves at least two different mechanisms Selection of Streptomyces ambofaciens mutants that produce large quantities of spiramycin and determination of optimal conditions for spiramycin production Genetic and biochemical features of spiramycin biosynthesis in Streptomyces ambofaciens. Curing, protoplast regeneration and plasmid transfer. Amplification of a particular DNA sequence in Streptomyces ambofaciens RP181110 reversibly prevents spiramycin production Dispensable ribosomal resistance to spiramycin conferred by srmA in the spiramycin producer Streptomyces ambofaciens The EMBL/GenBank accession number for the nucleotide sequence described in this paper is AJ223970. Regulation of the Biosynthesis of the Macrolide Antibiotic Spiramycin in <i>Streptomyces ambofaciens</i> Influence of short-chain fatty acids on the production of spiramycin by Streptomyces ambofaciens Genome mining of <i>Streptomyces ambofaciens</i>