Tropane alkaloids widely distributed in some genera of the Solanaceae family and decomposed bases were submitted to paper chromatography with various developing solvents, in comparison with hygrine, xanthine, and betaines, which are known to be fairly widely distributed in the Solanaceae and related families, and synthesized homatropine (Table I). In tropane alkaloids, there were marked differences between the original alkaloids and their degraded bases in their Rf values by various developing solvents and coloration to the Dragendorff reagent. Hygrine, xanthine, and betaines showed different Rf values from those of tropane alkaloids and their degraded bases. This paper chromatographic procedure enabled isolation of nortropine in a good yield from scopolia extract, and isolation and identification of scopine and scopoline from the hydrolyzate of scopolamine.Modification of the Dragendorff reagent for identification of tropane alkaloids by paper chromatography was examined. The use of hydrochloric acid in place of nitric acid and dilution with 1000 potassium iodide solution were found to give a formula which is stable over a long period and give comparatively quantitative result in examining the limit of alkaloidal coloration in paper partition chromatography. Limit of detection of alkaloids and betaines, and developed area of atropine in paper partition chromatography were examined by this modified reagent.