Heliosupine, heliosupine N-oxide, 3'-acetylheliosupine, and viridiflorine were isolated and identified on the base of MS, 1H and 13C NMR from Cynoglossum officinale. Altogether 14 pyrrolizidine alkaloids were separated and identified by GLC and GLC-MS in the alkaloid extracts from different parts of Cynoglossum officinale. From Cynoglossum amabile five pyrrolizidine alkaloids were recorded: supinine, amabiline, rinderine, echinatine, and 3'-O-acetylechinatine.