Two new C19-ditepenoid alkaloids, racemulolines A (1) and B (2), were isolated from the whole plants of Aconitum racemulosum Franch var. pengzhounense, and their structures were elucidated by spectral analysis. In continuation of our research in Aconitum racemulosum Franch var. pengzhounense W. J. Zhang et G. H. Chen, two C19-diterpenoid alkaloids, racemulolines A (1) and B (2), were isolated. In this paper, we report separation and structural elucidation of the new alkaloids (1~2). The new bases, racemulolines A (1) and B (2), were isolated as amorphous powder substances. Their molecular formulae, C24H39NO6 and C22H35NO4, respectively, were inferred from their HREIMS and 13C NMR spectra. Their NMR and MS spectra showed that they were the C19-diterpenoid alkaloids.