<jats:p> Further examination of the pithomycolide fraction of extracts of cultures of Pithomyceschartarum has resulted in the isolation of a new depsipeptide, for which the structure cyclo(L-N-methylalanyl-L-valyl-D-3-oxy-3-phenylpropionyl-D-3-oxy-3-phenylpropionyl-L-2-oxy-3-methylbutyryl) is proposed.