Among the cell lipids of Streptomyces toyocaensis, a lipoamino acid ester was observed in a relatively large amount, together with diphosphatidylglycerol and phosphatidylethanolamines. This amino acid containing lipid contains no phosphorus, but contains lysine and ornithine in a ratio of 9: 91. From a combination of mild alkali hydrolysis, acid hydrolysis, amino acid analysis, gas-liquid chromatographic and mass spectroscopic studies of the degradation products, the structure has been deduced as ethyleneglycol esterified with both N~-3-hydroxy fatty acyl-L-ornithine (or lysine) and 2-hydroxy fatty acids, like the ornithine lipid from Myeobaterium boris (B.C.G.). Also, the structure of siolipin previously reported is revised as having the same basic structure.