Twenty-five quinolizidine alkaloids, and the dipiperidyl alkaloid, ammodendrine, have been found in Petteria ramentacea. Nineteen alkaloids are described for the first time in this plant. Cytisine-12-carboxy-methylester represented a new quinolizidine alkaloid. The distribution of alkaloids in buds, leaves, flowers, ripe pods, unripe fruits, seeds, stem bark, stem wood and roots was investigated. The alkaloid content and alkaloid profile changed strongly during the vegetation period.