From the aerial parts of GENTIANA GELIDA two new strongly bitter acylsecoiridoid glucosides, gelidoside ( 1) and gentomoside ( 2), have been isolated along with five known secoiridoid glucosides, trifloroside ( 3), gentiopicroside ( 4), swertiamarin ( 5), eustomoside ( 6), and eustomorusside ( 7). The structures of the compounds were elucidated by a combination of spectroscopic methods (UV, IR, (1)H-NMR, 2D-NMR, EI- and FAB-MS).