A new ester alkaloid, (-)-3 beta-hydroxy-13 alpha-tigloyloxylupanine was isolated from the seedlings of C ytisus scoparius. Six known alkaloids, (-)-3 beta,13 alpha-dihydroxylupanine, (+)-13 alpha-tigloyloxylupanine, (+)-13 alpha-hydroxylupanine, (+)-lupanine, (-)-sparteine and(-)-17-oxosparteine were also identified by co-TLC, co-HPLC and GCMS with authentic samples. The structure of the new alkaloid was determined by spectroscopic methods. The new acyltransferase activity, tigloyl-CoA: 3 beta,13 alpha-dihydroxylupanine 13-O-tigloyltransferase (DHLTase) [EC 2.3.1.-], for the formation of this alkaloid was detected in the cell-free extracts of 15-day-old seedlings of C. scoparius.