The genus Cytisus L. has been revised recently in "FLORA EUROPEAE", and taxa spread in Bulgaria are referred to three genera: Chamaecytisus Link (20 species), Corothamnus (Koch) Presl (3 species) and Lembotropis Grijeb (1 species). Chamaecytisus austriacus is indigenous to the South and South-West regions of Bulgaria and is a rich source of sparteine (about 0.2%) with pharmaceutical industry interest. We investigated the alkaloid content of its air-dried leaves and fruits. The mixture of free bases was obtained by concentrating ethanol extract, acidifying with 5% HCl, removing nonbasic substances with ether, alkalizing with ammonia and extracting with ether (fraction 1), then treating with 10% NaOH (pH 13-14) and extracting with ether (fraction 2) and chloroform (fraction 3). Fraction 1 was subjected to silica gel column chromatography, yielding l-17 oxosparteine (identified by m.p., [α]D, IR spectrum, TLC) and l-sparteine. Fraction 2 was converted to hydrobromide and identified as l-sparteine. Fraction 3 contained sparteine, 17-oxosparteine, lupanine and an unknown alkaloid identified as lusitanine (via spectral data, m.p., [α]D, elemental analysis). Fruits extracted with methanol yielded 0.7% alkaloid mixture, with sparteine (91%) and lusitanine (1.16%) identified by gas chromatography.