Trichoflectin (1), a new azaphilone belonging to the deflectin series, 6-deoxy-7-0-demethyl-3,4-anhydrofusarubin (2), and 6-deoxy-3,4-anhydrofusarubin (3) were isolated from submerged cultures of the ascomycete Trichopezizella nidulus. All compounds showed antimicrobial activity and inhibited dihydroxynaphthalene melanin biosynthesis in fungi. The structure of trichoflectin (1) was elucidated by spectroscopic methods.