We continue our further research into the bioactive constituents of the ascomycete Tuber indicum, from which seven compounds, 5ξ-hydroxypyrrolidin-2-one (1), ergosterol peroxide (2), ergosterol (3), uracil (4), adenosine (5), azelaic acid (6), and D-allitol (7), have been isolated and structurally elucidated. The in vitro inhibitory activity toward phospholipase A2 (PLA2) was tested for these compounds. Anti-phospholipase A2 (PLA2) bioassay showed sterol 2 alone had in vitro selectively inhibitory activity against PLA2 from Crotalus adamenteus venom with an ED50 value of 100 µg/mL, but inactive against Apis mellifcra bee venom PLA2. Of these isolates, others except for 3 were obtained for the first time from the genus Tuber.