<jats:title>Abstract</jats:title><jats:p>Three new metabolites, asperfumigatin (<jats:bold>1</jats:bold>), isochaetominine (<jats:bold>10</jats:bold>), and 8′‐<jats:italic>O</jats:italic>‐methylasterric acid (<jats:bold>21</jats:bold>), together with nineteen known compounds, were obtained from the culture of <jats:italic>Aspergillus fumigatus</jats:italic>, an endophytic fungus from the Chinese liverwort <jats:italic>Heteroscyphus tener</jats:italic> (<jats:sc>Steph.) Schiffn.</jats:sc> Their structures were established by extensive analysis of the spectroscopic data. The absolute configurations of <jats:bold>1</jats:bold> and <jats:bold>10</jats:bold> were determined by analysis of their respective CD spectra. Cytotoxicity of these isolates against four human cancer cell lines was also determined.