<jats:title>Abstract</jats:title><jats:p>Three new sphingosine‐type glucocerebrosides, CE‐2b (<jats:bold>1</jats:bold>), CE‐2c (<jats:bold>2</jats:bold>) and CE‐2d (<jats:bold>3</jats:bold>), were isolated by means of highperformance liquid chromatography (HPLC) from a cerebroside molecular species (CE‐2) which was obtained from the less polar fraction of the methanol extract of the sea cucumber <jats:italic>Cucumaria echinata</jats:italic>. The structures of these cerebrosides were determined on the basis of chemical and spectroscopic evidences. <jats:chem-struct-wrap><jats:chem-struct><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/gif" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/must001.gif"><jats:alt-text>magnified image</jats:alt-text></jats:graphic></jats:chem-struct></jats:chem-struct-wrap>