The α‐Ketoglutarate/FeII‐Dependent Dioxygenase VldW Is Responsible for the Formation of Validamycin B

ChemBioChem
2012.0

Abstract

From A to B: Through detailed biochemical investigations, we discovered that VldW, an α-ketoglutarate/Fe(II)-dependent dioxygenase, regioselectively hydroxylates validamycin A to validamycin B. The results provide insights into the biosynthesis of hydroxylated validamycins and could be used to control the metabolic outcomes of the validamycin pathway.

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