As we reported previously1'2), amidepsines A to D were isolated from the culture broth of Humicola sp. FO-2942 as inhibitors of diacylglycerol acyltransferase3) (acyl-CoA: 1,2-diacyl-^-glycerol O-acyltransferase, abbreviated as DGAT)[EC 2.3.1.20]. In the course of our continuous screening, a fungal strain FO-5969 was found to produce different members of amidepsines. Amidepsine A and a new compoundnamed amidepsine E (Fig. 1), were isolated from the culture broth of the strain. Amidepsine E was not detected in the FO-2942 culture broth prepared under the reported condition'1*. In this paper, the taxonomy of the producing strain, fermentation, isolation, biological properties and struc-ture elucidation of amidepsine E are described.