In this paper, we describe the isolation and structural determination of 2,7-dihydroxyapogeissoschizine, a new alkaloid from the root bark of Strychnos gossweileri. Elucidation of its structure is based mainly on 1D and 2D NMR studies; its conformation was optimized by energy minimization. According to a preliminary test, this substance shows low toxicity to B16 melanoma cells, but not for non-cancer 3T3 fibroblasts cultured in vitro.