The novel compounds, astronypyrone, astronyquinone, and astronyurea, together with three known compounds, xestodecalactone A, ent-coryoctalactone B, and dimethoxy-O-methylpulvinone were isolated from the marine-derived fungus Astrosphaeriella nypae BCC 5335. Their structures were elucidated on the basis of NMR spectroscopy and mass spectrometry data. Astronypyrone possesses a 2-pyrone derivative fused with a tetrahydrofuran moiety via a dihydropyran unit, while the 2-pyrone ring is substituted by a naphthoquinone skeleton in astronyquinone.