A 4'-O-sulphated analogue of wedeloside, a new flavanone, 7,4'-dihydroxy-8,3'-dimethoxyflavanone and its related chalcone, together with the 4'-O-β-D-glucopyranoside of the latter, and a number of known compounds were isolated from the aerial parts of Wedelia asperrima. Their structures were established by spectroscopic techniques and by synthesis of the flavanone and chalcone.