New three conjugated serotonins were isolated from safflower meal (carthamus tinctorius L.). On the basis of spectral properties and chemical evidence, their structures were determined as N-feruloylserotonin (4), N-(p-coumaroyl)serotonin (5) and N-(p-coumaroyl)serotonin mono-ƒÀ-D-glucopyranoside (6). The known compounds, 2-hydroxyarctiin (2), matairesinol mono-ƒÀ-D-glucoside (1) and acacetin (3) were also isolated and identified.