A new peptide alkaloid designated as anorldianine [I] has been isolated from the CHCI, extract of the stern bark of Canthirrnr anoddianuni and characterized by spectroscopic methods. Canthium anwldianum De Wild & Th. Dur (1) (Rubiaceae) is one of 230 Canthium species (2) widely distributed throughout the world. The genus is known for its folkloric applications against a number of ailments such as cough, diabetes, and hypertension (3,4). Previous studies (5-10) on the genus reported the isolation of triterpenes, coumarins, iridoids, and a peptide alkaloid. As part ofour contribution to the study of the family Rubiaceae, we now report the isolation and characterization of a new peptide alkaloid named anorldianine El) from C. anorldianum.