Nine new pentacyclic polyketides, fasamycins G-K (<b>1</b>-<b>5</b>) and formicamycins N-Q (<b>6</b>-<b>9</b>), along with 10 known analogues (<b>10</b>-<b>19</b>), were isolated from a rhizospheric soil-derived <i>Streptomyces</i> sp. KIB-1414. Their structures and absolute configurations were elucidated by interpretation of NMR and HRMS data and comparisons of CD data. The compounds were active against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), <i>Staphylococcus aureus</i>, <i>Bacillus subtilis</i>, and <i>Escherichia coli</i> strains, with MIC values ranging from 0.20 to 50.00 μg/mL.