<jats:p><jats:bold>Abstract: </jats:bold> Radishes (<jats:italic>Raphanus sativus</jats:italic> L.) are members of the cruciferous vegetable family that contain many classes of biologically active phytochemicals. This study determined the phytochemical composition of the sprouts and mature taproots of 8 radish varieties. Radish sprouts contained significantly greater concentrations of glucosinolates (3.8‐fold) and isothiocyanates (8.2‐fold) than the mature radish taproot and also contained significantly greater concentrations of phenolics (on average 6.9‐fold). The anthocyanin concentrations of the mature radish taproot were significantly greater than in the sprouts of red, pink, and purple varieties. The primary anthocyanidins present in the red and pink radish varieties were pelargonidin and delphinidin, while the primary anthocyanidin in the purple radish variety was cyanidin. Radish sprouts were between 9‐ and 59‐fold more potent than the corresponding mature taproot at activating the antioxidant response element (ARE) in a stably transfected hepatoma cell line. The ARE activity of the radish sprouts and mature taproots was significantly correlated with the total isothiocyanate concentration of the radishes.<jats:p><jats:bold>Practical Application: </jats:bold> Understanding the influence variety and developmental stage has on the biological activity of cruciferous vegetables provides important information for further studies examining the <jats:italic>in vivo</jats:italic> effects of radish treatment and foundation for providing recommendations to reduce the risk of chronic disease through dietary intervention.