The culture ®ltrate of Fusarium oxysporum f sp matthiolae was inspected on the occurrence of jasmonates and related compounds. Among compounds described for the ®rst time of biological origin are 7-iso-cucurbic acid, (1S,2S)- and (1S,2R)-3 oxo-2-pentylcyclopentane-1-butyric acid, (1S,2S)- and (1S,2R)-3-oxo-2-(2Z-pentenyl)cyclopentane-1-hexanoic acid, (1S,2S)- and (1S,2R)-3-oxo-2-pentylcyclopentane-1-hexanoic acid, (1S,2S)-3-oxo-2-(2Z-pentenyl)cyclopentane-1-octanoic acid, (1S,2S)-3-oxo-2-pentylcyclopentane-1-octanoic acid and N-[9,10-dihydro-7-iso-jasmonoyl]-(S)-isoleucine. The following metabolites were identi®ed for the ®rst time for this fungus: (ÿ)-Jasmonic acid, 9,10-dihydrojasmonic acid and N-[(ÿ)-jasmonoyl-(S)]-isoleucine were major constituents of the culture ®ltrate, whereas as minor metabolites occurred N-[9,10-dihydrojasmonoyl]-(S)-isoleucine, cucurbic acid and 3-oxo-2-(2Z-pentenyl)cyclopentane-1-butyric acid, 3-oxo-2-(2Z-pentenyl)cyclopentane-1-octanoic acid and 3 oxo-2-pentylcyclopentane-1-octanoic acid. All cyclopentanones found carried a cis- or trans-attached side chain. Didehydrojasmonates, hydroxylated jasmonates or 12-oxophytodienoic acid could not be detected in the culture ®ltrate.