Expression of the Streptomyces alboniger pur cluster in Streptomyces lividans is dependent on the bldA‐encoded tRNALeu

FEBS Letters
1998.0

Abstract

<jats:p> <jats:italic>Streptomyces lividans</jats:italic> 1326‐9, a <jats:italic>bldA</jats:italic> <jats:sup>+</jats:sup> strain, and its <jats:italic>bldA39</jats:italic> mutant derivative J1725 were transformed with a cosmid containing the <jats:italic>pur</jats:italic> cluster, which determines the puromycin biosynthetic pathway from <jats:italic>Streptomyces alboniger</jats:italic>. <jats:italic>bldA</jats:italic> <jats:sup>+</jats:sup> transformants produced puromycin in typical amounts, whereas <jats:italic>bldA39</jats:italic> transformants did so at drastically decreased levels. Transformation of low producers with the wild‐type <jats:italic>bldA</jats:italic> gene reverted this phenotype to normal production. These data, in addition to the presence of a TTA codon in the amino‐terminal coding region of the <jats:italic>pur10</jats:italic> and <jats:italic>pur6</jats:italic> genes of the <jats:italic>pur</jats:italic> cluster, suggest that the puromycin biosynthetic pathway is translationally dependent on the <jats:italic>bldA</jats:italic> gene product, a tRNA<jats:sup>Leu</jats:sup>.

DOI： 10.1016/s0014-5793(97)01564-0

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