The Isolation of a NewS-Methyl Benzothioate Compound from a Marine-DerivedStreptomycessp.

Abstract

<jats:p>The application of an HPLC bioactivity profiling/microtiter plate technique in conjunction with microprobe NMR instrumentation and access to the AntiMarin database has led to the isolation of a new<jats:bold>1</jats:bold>. In this example,<jats:bold>1</jats:bold>was isolated from a cytotoxic fraction of an extract obtained from marine-derived<jats:italic>Streptomyces</jats:italic>sp. cultured on Starch Casein Agar (SCA) medium. The 1D and 2D<jats:sup>1</jats:sup>H NMR and ESIMS data obtained from 20 <jats:italic>μ</jats:italic>g of compound<jats:bold>1</jats:bold>fully defined the structure. The known<jats:bold>2</jats:bold>was also isolated and readily dereplicated using this approach.