A cyclic lipodepsipeptide, verruculin, a rosigenin and an aposphaerin analogues, verruculinone and 7-Omethylaposphaerin C, together with six known compounds were isolated from the marine fungus Verruculina enalia BCC 22226. The structures of these compounds were elucidated by extensive spectroscopic analysis. The absolute configuration of verruculin was determined by Marfey's method, and the relative configuration of verruculinone was obtained by X-ray crystallography. Verruculin showed weak antituberculosis and antibacterial activities, while verruculinone displayed cytotoxicity against cancer cell (MCF-7 and NCIeH187) and Vero cell lines.