Two new 14-membered frangulanine-type cyclopeptide alkaloids, lotusanines A (1) and B (2), have been isolated from the aerial parts of Ziziphus lotus, together with the known alkaloids sanjoinenine (3), sanjoinine F (4), and frangufoline (5). Ziziphus lotus Lam. (Rhamnaceae) is a small tree that is widely distributed in the Jordan Valley. The genus Ziziphus comprises several species that are used in indigenous medicine for the treatment of various diseases (1-5). We report here the first chemical investigations of Z. lotus, yielding two new 14-membered frangulanine-type (6) cyclopeptide alkaloids from an extract of the aerial parts of Z. lotus. The new compounds, lotusanine A (1) and lotusanine B (2) were isolated along with the known alkaloids, sanjoinenine (3) (7), sanjoinine F (4) (7), and frangufoline (5) (8) (Table 1). Characterization of these compounds was achieved with the help of spectroscopic studies, especially mass spectrometry, which permitted conclusions to be drawn about the structural units (aminoacids and styryl moiety) present in the compound and the manner in which they are linked (6,9).