<jats:title>Abstract</jats:title><jats:p>Chemical Screening with extracts of Actinomycetes strains resulted in the detection, isolation, and structural elucidation of two new heteroaromatic secondary metabolites, named ulupyrinone (<jats:bold>1</jats:bold>) and ulufuranol (<jats:bold>2</jats:bold>), from <jats:italic>Streptomyces spina</jats:italic> (strain FH‐S 2144). The absolute configuration of ulufuranol (<jats:bold>2</jats:bold>) was determined by analysis of the CD spectra of its dibenzoate, <jats:bold>3</jats:bold>, using the dibenzoate chirality rule.