In the course of our screening program for plant growth inhibitors, Streptomyces sp. K385 isolated from a soil sample collected in Okayama Japan, was found to produce kitamycin A (1) and B (2), new antimycin type antibiotics, together with known antimycin type antibiotics, antimycin A1, A2a, A3, A4a, urauchimycin B (3), and deisovalerylblastmycin (4). This study describes the fermentation, isolation, structure elucidation and biological activity of 1 and 2 and stereochemistry of 3.