Oxytropis myriophylla (Leguminosae) is a medicinal plant growing wild in southeast and northwest areas of China. It has been used as a folk medicine in China for the treatment of cold and inflammation of carbuncle swelling, pain and different types of bleeding. Studies on the chemical constituents and bioactivities have not been reported so far. In our recent research, a 95% EtOH extract of the plant was separated by repeated chromatography to give eight compounds. On the basis of spectral analyses, their structures were elucidated to be (6R,9R)-roseoside (1), (6R,9S)-roseoside (2), adenosine (3), quercetin-7-O-a-L-rhamnopyranosyl-3-O-(60-P-coumaroyl)-b-D-glucopyranosyl-(1→2)-b-D-xylopyranoside (4), quercetin-7-O-a-L-rhamnopyranosyl-3-O- (60-caffeoyl)-b -D-Glucopyranosyl-(1→2)-b -D-xylopyranoside (5), kaempferol-7-O-a-L-rhamnopyranosyl-3-O-(60- P-coumaroyl)-b -D-glucopyranosyl-(1→2)-b -D-xylopyranoside (6), quercetin-7-O-a-L-rhamnopyranosyl-3-O-(60-feruloyl)-b-D-glucopyranosyl-(1→2)-b-D-xylopyranoside (7), quercetin-7-O-a -L-rhamnopyranos-yl-1-3-O-(60-Pcoumaroyl)-b-D-glucopyranosyl-(1→2)-b-D-glucopyranoside (8). Five flavonoids (4—8, Fig. 1) were new compounds called myriophylloside B (4), myriophylloside C (5), myriophylloside D (6), myriophylloside E (7), and myriophylloside F (8), and the known compounds were isolated from this plant for the first time.