In the course of screening for antimicrobial metabolites of basidiomycetes, piptamine 1 was isolated from a submerged culture of Piptoporus betulinus Lu 9-1. This study reports the production, isolation, structure elucidation, and biological activity of 1. The strain was isolated from a forested district near Neustadt/Orla (Thuringia, Germany). Cultivation was performed in a seed medium at 23°C and 110 rpm for 21 days. The broth was extracted with ethyl acetate, and purification via Sephadex LH-20 and silica gel 60 column chromatography yielded 35 mg of 1 as a waxy solid. HRESI-MS determined the molecular formula C23H41N, and 1D/2D NMR (COSY, DEPT, HSQC, HMBC) confirmed 1 as a hitherto unknown fungal tertiary amine. Antimicrobial activity tests showed that piptamine 1 is active against Gram-positive bacteria, yeasts, and fungi, with notable MIC values against Staphylococcus aureus SG 511 (0.78 μg/ml) and Enterococcus faecalis 1528 (1.56 μg/ml). It exhibited lower activity against Gram-negative bacteria (e.g., Escherichia coli SG 458, MIC 12.5 μg/ml) and Penicillium notatum JP 36 (>50 μg/ml).