Isolation and structure determination of a new lasso peptide subterisin from Sphingomonas subterranea

Tetrahedron Letters
2017.0

Abstract

A new lasso peptide named subterisin was isolated from the culture broth of Sphingomonas subterranea NBRC 16086T . The molecular formula of subterisin was established as C78H121O22N21 based on accurate mass analysis. The chemical structure of subterisin was determined by 2D NMR experiments. The presence of macrolactam ring of Gly1–Glu8 was indicated by NOESY experiment and MS/MS analysis. The three-dimensional structure of subterisin in solution was established by calculation based on NMR data. The proposed biosynthetic gene cluster of subterisin was found on the genome of S. subterranea.

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