Chemical investigation of the ethanolic extracts of the whole plant of Ophioglossum pedunculosum afforded seven new homoflavonoid glucosides, pedunculosumosides A-G (1-7). Pedunculosumosides A and C exhibit modest activity of blocking HBsAg secretion with IC(50) values of 238.0 and 70.5 μM, respectively.